A simple, specific, accurate, and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Itraconazole in capsule dosage form. A dionex C-18, 5 µm column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing Potassium dihydrogen phosphate buffer solution and Methanol in the ratio of 60:40 v/v was used. The flow rate was 1.5 ml/min and effluents were monitored at 306 nm. The retention time of Itraconazole was found to be 5.2 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification was found to be 0.0018594 mg/ml and 0.006197 mg/ml respectively and recovery of Itraconazole from capsule formation was found to be 99 to 100%. The system suitability parameters such as theoretical plates and tailing factor were found to be 8609 and 1.13. The proposed method was successfully applied to the quantitative determination of Itraconazole in capsule formulation.
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